{"id":57142,"date":"2016-06-19T22:13:00","date_gmt":"2016-06-19T22:13:00","guid":{"rendered":"http:\/\/127.0.0.1:10081\/?p=57142 "},"modified":"2016-06-19T22:13:00","modified_gmt":"2016-06-19T22:13:00","slug":"57142-revision-v1","status":"publish","type":"post","link":"https:\/\/minzhuzhongguo.org\/?p=57142","title":{"rendered":"\u201c\u4e1c\u65b9\u8bfa\u8d1d\u5c14\u5956\u201d\u5510\u5956\u516c\u5e03\u4eca\u5e74\u5f97\u4e3b"},"content":{"rendered":"<p style=\"-webkit-font-smoothing: antialiased; padding: 0px 40px 12px 0px; font-size: 19px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; background-color: #ffffff;\">\u53f0\u6e7e\u5a92\u4f53\u79f0\u5510\u5956\u4e3a\u201c\u4e1c\u65b9\u8bfa\u8d1d\u5c14\u5956\u201d\uff0c\u5176\u5956\u91d1\u91d1\u989d\u6bcf\u9879\u9ad8\u8fbe5000\u4e07\u65b0\u53f0\u5e01\u30022014\u5e74\u7684\u5510\u5956\u5f97\u4e3b\u5305\u62ec\u53f2\u5b66\u5927\u5e08\u4f59\u82f1\u65f6\u3002<\/p><div full\"=\"\" style=\"margin: 7px 0px 10px; padding: 7px 20px 10px 0px; float: none; width: 700px; clear: both; font-family: Simsun; font-size: medium; background-color: #ffffff;\"><a init\"=\"\" href=\"http:\/\/www.dw.com\/zh\/%E4%B8%9C%E6%96%B9%E8%AF%BA%E8%B4%9D%E5%B0%94%E5%A5%96%E5%94%90%E5%A5%96%E5%85%AC%E5%B8%83%E4%BB%8A%E5%B9%B4%E5%BE%97%E4%B8%BB\/a-19340981#\" link=\"\/overlay\/overlay_image\/s-1686\/c-19340981\/18996237\" rel=\"nofollow\" style=\"color: #0087eb; text-decoration: none; cursor: pointer;\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"394\" border=\"0\" src=\"http:\/\/www.dw.com\/image\/0,,18996237_303,00.jpg\" alt=\"Bildkombo Emmanuelle Charpentier Jennifer A. Doudna\" title=\"\u5510\u59562016\u5e74\u751f\u6280\u533b\u836f\u5956\u4e09\u4f4d\u5f97\u4e3b\u4e2d\u7684\u6cd5\u56fd\u4eba\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\u548c\u7f8e\u56fd\u4eba\u73cd\u59ae\u5f17\u00b7\u9053\u7eb3\" style=\"margin: 0px; padding: 0px; width: 700px;\" \/><\/a><p style=\"-webkit-font-smoothing: antialiased; color: #888888; padding: 4px 0px; font-size: 15px; line-height: 20px; font-family: 'MS Song', 'MS Hei'; width: 720px;\">\u5510\u59562016\u5e74\u751f\u6280\u533b\u836f\u5956\u4e09\u4f4d\u5f97\u4e3b\u4e2d\u7684\u6cd5\u56fd\u4eba\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\u548c\u7f8e\u56fd\u4eba\u73cd\u59ae\u5f17\u00b7\u9053\u7eb3<\/p><\/div><div style=\"margin-bottom: 30px; font-family: Simsun; font-size: medium; background-color: #ffffff;\"><div style=\"-webkit-font-smoothing: antialiased; color: #3e3e3e; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei';\"><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\uff08\u5fb7\u56fd\u4e4b\u58f0\u4e2d\u6587\u7f51\uff09\u5510\u5956\u4e8e2012\u5e7412\u6708\u7531\u53f0\u6e7e\u4f01\u4e1a\u5bb6\u5c39\u884d\u6a11\u6210\u7acb\uff0c\u8bbe\u7f6e\u6c38\u7eed\u53d1\u5c55\u3001\u751f\u6280\u533b\u836f\u3001\u6c49\u5b66\u53ca\u6cd5\u6cbb\u56db\u5927\u5956\u9879\u3002\u81ea2014\u5e74\u8d77\uff0c\u5510\u5956\u6bcf\u4e24\u5e74\u9881\u53d1\u4e00\u6b21\uff0c\u63d0\u4f9b\u6bcf\u4e00\u5956\u9879\u5956\u91d1\u65b0\u53f0\u5e014000\u4e07\u5143\uff08\u7ea6\u5408124\u4e07\u7f8e\u5143\uff09 \uff0c\u5e76\u63d0\u4f9b\u5f97\u5956\u4eba\u7814\u7a76\u8865\u52a9\u8d39\u65b0\u53f0\u5e011000\u4e07\u5143\uff08\u7ea6\u540831.1\u4e07\u7f8e\u5143\uff09\u3002\u6bcf\u5c4a\u6bcf\u4e00\u5956\u9879\u4e4b\u5f97\u5956\u4eba\u6700\u591a\u4e3a\u4e09\u4eba\u3002\u53f0\u6e7e\u4e2d\u592e\u7814\u7a76\u9662\u8d1f\u8d23\u56db\u5927\u5956\u9879\u7684\u63d0\u540d\u548c\u8bc4\u9009\u3002\u4eca\u5e74\u7684\u5510\u5956\u5f97\u4e3b\u540d\u5355\u75311986\u5e74\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\u5f97\u4e3b\u3001\u4e2d\u592e\u7814\u7a76\u9662\u524d\u9662\u957f\u674e\u8fdc\u54f2\u5ba3\u5e03\u3002\u56db\u5927\u5956\u9879\u82b1\u843d\u8c01\u5bb6\uff0c\u5206\u522b\u57286\u670818\u65e5\u30016\u670819\u65e5\u30016\u670820\u65e5\u548c6\u670821\u65e5\u516c\u5e03\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u5510\u59566\u670819\u65e5\u516c\u5e03\u751f\u6280\u533b\u836f\u5956\u5f97\u4e3b\uff0c\u7531\u6cd5\u56fd\u7684\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\uff08Emmanuelle Charpentier\uff09\u3001\u7f8e\u56fd\u7684\u73cd\u59ae\u5f17\u00b7\u9053\u7eb3\uff08Jennifer A. Doudna\uff09\u4e0e\u534e\u88d4\u7f8e\u7c4d\u7684\u5f20\u950b\uff08Feng Zhang\uff09\u4e09\u4eba\u5171\u540c\u83b7\u5f97\uff0c\u8868\u5f70\u5176\u5728CRISPR\/Cas9\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u4e0a\u7684\u8d21\u732e\uff0c\u5c06\u5927\u5e45\u6539\u9769\u751f\u533b\u7814\u7a76\u4e0e\u75be\u75c5\u6cbb\u7597\u7684\u7b56\u7565\uff0c\u6781\u5177\u6f5c\u529b\u6cbb\u7597\u4eba\u7c7b\u5404\u79cd\u75be\u75c5\u3002<\/p><div style=\"margin: 0px; padding: 7px 20px 10px 0px; float: left; width: 340px;\"><a init\"=\"\" href=\"http:\/\/www.dw.com\/zh\/%E4%B8%9C%E6%96%B9%E8%AF%BA%E8%B4%9D%E5%B0%94%E5%A5%96%E5%94%90%E5%A5%96%E5%85%AC%E5%B8%83%E4%BB%8A%E5%B9%B4%E5%BE%97%E4%B8%BB\/a-19340981#\" link=\"\/overlay\/overlay_image\/s-1686\/c-19340981\/19340942\" rel=\"nofollow\" style=\"color: #0087eb; text-decoration: none; cursor: pointer;\"><img loading=\"lazy\" decoding=\"async\" width=\"340\" height=\"191\" border=\"0\" src=\"http:\/\/www.dw.com\/image\/0,,19340942_404,00.jpg\" alt=\"Labor in Berkeley Designer Babies\" title=\"\u73cd\u59ae\u5f17\u00b7\u9053\u7eb3\uff08Jennifer A. Doudna\uff0c \u53f3\uff09\" style=\"margin: 0px; padding: 0px; width: 340px;\" \/><\/a><p style=\"-webkit-font-smoothing: antialiased; color: #888888; padding: 4px 0px; font-size: 15px; line-height: 20px; font-family: 'MS Song', 'MS Hei';\">\u73cd\u59ae\u5f17\u00b7\u9053\u7eb3\uff08Jennifer A. Doudna\uff0c \u53f3\uff09<\/p><\/div><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u7406\u89e3\u5404\u522b\u57fa\u56e0\u7684\u529f\u80fd\u4e0e\u4ed6\u4eec\u4e4b\u95f4\u4e92\u52a8\u7684\u6a21\u5f0f\uff0c\u662f\u89e3\u5f00\u751f\u547d\u8c1c\u56e2\u7684\u5173\u952e\uff0c\u4f7f\u4eba\u7c7b\u5f97\u4ee5\u53d1\u5c55\u75be\u75c5\u6cbb\u7597\u7b56\u7565\u3002\u957f\u4e45\u4ee5\u6765\uff0c\u79d1\u5b66\u5bb6\u68a6\u60f3\u6709\u671d\u4e00\u65e5\u53ef\u4ee5\u7cbe\u51c6\u6709\u6548\u7387\u5730\u4fee\u6b63\u7279\u5b9a\u57fa\u56e0\uff0c\u5e76\u4e14&#8221;\u7f16\u8f91&#8221;\u5254\u9664\u6709\u7f3a\u9677\u7684\u57fa\u56e0\u3002\u611f\u8c22\u4eca\u5e74\u5510\u5956\u751f\u6280\u533b\u836f\u5956\u5f97\u4e3b\u4eec\uff0c&#8221;\u6709\u671d\u4e00\u65e5&#8221;\u4fbf\u662f&#8221;\u4eca\u65e5&#8221;\u3002\u8fc7\u53bb\u66fe\u53d1\u5c55\u6570\u79cd\u65b9\u5f0f\u53ef\u4ee5\u8fdb\u884c\u57fa\u56e0\u4f53\u7f16\u8f91\uff0c\u4f46\u662f\u7531\u8fd9\u4e09\u4f4d\u5f97\u5956\u8005\u6240\u5f00\u53d1\u7684CRISPR\/Cas9\u5e73\u53f0\uff0c\u5728\u6548\u7387\u3001\u7cbe\u51c6\u5ea6\u548c\u654f\u9510\u5ea6\u4e0a\uff0c\u5927\u80dc\u4ee5\u5f80\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u590f\u5f6d\u63d0\u8036\u535a\u58eb\u548c\u9053\u7eb3\u535a\u58eb\u5c06\u539f\u672c\u7ec6\u83cc\u4e2d\u7684CRISPR\/Cas9\u7cfb\u7edf\uff0c\u7b80\u5316\u6210\u6613\u4e8e\u64cd\u4f5c\u3001\u53ef\u4ee5\u7a0b\u5f0f\u5316\u7684\u53cc\u5206\u5b50\u7cfb\u7edf\uff0c\u4f7f\u57fa\u56e0\u4f53\u7f16\u8f91\u66f4\u6709\u6548\u7387\u3002\u540c\u4e00\u65f6\u95f4\uff0c\u5f20\u950b\u535a\u58eb\u5bf9Cas9\u7cfb\u7edf\u8fdb\u884c\u81ea\u5df1\u72ec\u7acb\u7684\u6539\u826f\u5b9e\u9a8c\uff0c\u7387\u5148\u5728\u54fa\u4e73\u7c7b\u548c\u4eba\u7c7b\u7ec6\u80de\u8fdb\u884c\u6210\u529f\u7684\u57fa\u56e0\u4f53\u7f16\u8f91\u3002\u800c\u540e\u66f4\u8fdb\u4e00\u6b65\u6539\u826f&#8221;\u540c\u65f6\u7f16\u8f91\u591a\u4e2a\u57fa\u56e0&#8221;\u3001\u4ee5\u53ca\u300c\u540c\u6e90\u6027\u57fa\u56e0\u4fee\u590d\u300d\u7684\u5b9e\u9a8c\u65b9\u6cd5\u3002\u8fd9\u4e09\u4f4d\u6770\u51fa\u79d1\u5b66\u5bb6\u7684\u6210\u5c31\uff0c\u5927\u5e45\u6539\u9769\u4e86\u57fa\u56e0\u4f53\u7f16\u8f91\u5e73\u53f0\uff0c\u4f7f\u5176\u7a0b\u5f0f\u5316\u3001\u666e\u53ca\u5316\u3001\u5e76\u4e14\u5f97\u4ee5\u91cf\u5316\u3002\u81ea\u4ece2012\u548c2013\u5e74\u520a\u51fa\u4ee5\u6765\uff0c\u5168\u7403\u4e0a\u767e\u4e43\u81f3\u4e0a\u5343\u95f4\u5b9e\u9a8c\u5ba4\u7686\u5229\u7528\u6b64\u5e73\u53f0\u8fdb\u884c\u4eba\u7c7b\u53ca\u5404\u79cd\u751f\u7269\u7ec6\u80de\u7684\u9057\u4f20\u5de5\u7a0b\uff0c\u5305\u62ec\uff1a\u6591\u9a6c\u9c7c\u3001\u690d\u7269\u3001\u7334\u5b50\u3001\u732a\u548c\u5c0f\u9f20\u3002\u8bb8\u591a\u4eba\u76f8\u4fe1\u8fd9\u662f\u7ee7\u57fa\u56e0\u9009\u6b96\u6280\u672f\uff08cloning\uff09\u548c\u805a\u5408\u9176\u8fde\u9501\u53cd\u5e94\uff08PCR\uff09\u4ee5\u6765\uff0c\u57fa\u56e0\u4f53\u7814\u7a76\u53f2\u4e0a\u6700\u4f1f\u5927\u7684\u79d1\u6280\u53d1\u5c55\u4e4b\u4e00\u3002\u56e0\u6b64\uff0c\u8fd9\u4e09\u4f4d\u79d1\u5b66\u5bb6\u83b7\u9009\u672c\u5c4a\u751f\u6280\u533b\u836f\u5956\u9879\u7684\u5171\u540c\u5f97\u5956\u4eba\uff0c\u4ed6\u4eec\u5c06\u4e00\u8d77\u5e73\u52064\u5343\u4e07\u5956\u91d1\u548c1\u5343\u4e07\u7684\u6307\u5b9a\u7814\u7a76\u63a8\u5e7f\u7ecf\u8d39\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u5510\u5956\u8bc4\u9009\u59d4\u5458\u4f1a\u8868\u793a\uff0c CRISPR\/Cas9\u7cfb\u7edf\u5df2\u6539\u53d8\u4eba\u4eec\u505a\u9057\u4f20\u5b9e\u9a8c\u7684\u65b9\u6cd5\uff0c\u66f4\u662f\u5c06\u7814\u53d1\u7684\u95f8\u95e8\u5927\u5f00\uff0c\u9020\u798f\u4eba\u7c7b\u7684\u75be\u75c5\u65b0\u7597\u6cd5\uff0c\u5c06\u5982\u6ed4\u6ed4\u6c5f\u6c34\uff0c\u8fde\u7ef5\u4e0d\u7edd\uff0c\u5c06\u6765\u751a\u81f3\u6709\u673a\u4f1a\u7528\u5728\u65b0\u751f\u7269\u80fd\u6e90\u7684\u5f00\u53d1\u6216\u4f5c\u7269\u7684\u6539\u826f\u3002\u65e0\u5eb8\u7f6e\u7591\uff0c\u6b64\u4e09\u4eba\u7684\u6210\u5c31\u4e0e\u8d21\u732e\uff0c\u5c06\u9020\u798f\u5168\u4f53\u4eba\u7c7b\u5065\u5eb7\u4e0e\u793e\u4f1a\u798f\u5740\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u751f\u6280\u533b\u836f\u83b7\u5956\u8005\u80cc\u666f\u8d44\u6599\uff1a<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\u535a\u58eb\uff0c\u6cd5\u56fd\u7c4d\u5fae\u751f\u7269\u5b66\u5bb6\uff0c\u5728\u75c5\u539f\u7ec6\u83cc\u7684\u611f\u67d3\u4e0e\u514d\u75ab\u8c03\u63a7\u673a\u5236\u7814\u7a76\u4e0a\uff0c\u88ab\u516c\u8ba4\u4e3a\u4e16\u754c\u9996\u5c48\u4e00\u6307\u7684\u4e13\u5bb6\u3002\u73b0\u804c\u4e3a\u5fb7\u56fd\u9a6c\u514b\u65af\u2027\u666e\u6717\u514b\u4f20\u67d3\u75c5\u751f\u7269\u5b66\u7814\u7a76\u6240\uff08Max Planck Institute for Infection Biology\uff09\uff0c\u4f20\u67d3\u75c5\u751f\u7269\u5b66\u8c03\u63a7\u90e8\u95e8\u7684\u90e8\u957f\u3002\u5728CRISPR\u7684\u53d1\u5c55\u4e2d\uff0c\u5176\u4e3b\u8981\u7684\u8d21\u732e\u5728\u4e8e\u53d1\u73b0Cas9\u86cb\u767d\u7684\u6d3b\u6027\u4ef0\u8d56tracrRNA\u3002<\/p><div style=\"margin: 0px; padding: 7px 20px 10px; float: right; width: 340px;\"><a init\"=\"\" href=\"http:\/\/www.dw.com\/zh\/%E4%B8%9C%E6%96%B9%E8%AF%BA%E8%B4%9D%E5%B0%94%E5%A5%96%E5%94%90%E5%A5%96%E5%85%AC%E5%B8%83%E4%BB%8A%E5%B9%B4%E5%BE%97%E4%B8%BB\/a-19340981#\" link=\"\/overlay\/overlay_image\/s-1686\/c-19340981\/19340945\" rel=\"nofollow\" style=\"color: #0087eb; text-decoration: none; cursor: pointer;\"><img loading=\"lazy\" decoding=\"async\" width=\"340\" height=\"191\" border=\"0\" src=\"http:\/\/www.dw.com\/image\/0,,19340945_404,00.jpg\" alt=\"Die Forscherin Emmanuelle Charpentier\" title=\"\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\uff08Emmanuelle Charpentier\uff09\" style=\"margin: 0px; padding: 0px; width: 340px;\" \/><\/a><p style=\"-webkit-font-smoothing: antialiased; color: #888888; padding: 4px 0px; font-size: 15px; line-height: 20px; font-family: 'MS Song', 'MS Hei';\">\u4f0a\u66fc\u7ebd\u00b7\u590f\u5f6d\u63d0\u8036\uff08Emmanuelle Charpentier\uff09<\/p><\/div><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u73cd\u59ae\u4f5b\u00b7\u9053\u7eb3\u535a\u58eb\u76ee\u524d\u5728\u7f8e\u56fd\u52a0\u5dde\u5927\u5b66\u67cf\u514b\u83b1\u5206\u6821\uff08University of California, Berkeley\uff09\uff0c\u62c5\u4efb\u5316\u5b66\u53ca\u5206\u5b50\u66a8\u7ec6\u80de\u751f\u7269\u5b66\u7cfb\u6559\u6388\uff0c\u662f\u970d\u534e\u5fb7\u2027\u4f11\u65af\u533b\u5b66\u7814\u7a76\u6240\uff08HHMI\uff09\u7684\u7814\u7a76\u5458\uff0c\u540c\u65f6\u4e5f\u662f\u7f8e\u56fd\u56fd\u5bb6\u79d1\u5b66\u9662\u9662\u58eb\u3002\u8eab\u4e3a\u7740\u540d\u7684RNA\u7ed3\u6784\u751f\u7269\u5b66\u5bb6\uff0c\u4ed6\u66fe\u7814\u7a76\u6838\u9176\u3001\u5185\u8f6c\u8bd1\u5b50\uff08IRES\uff09\u3001\u548c\u8f83\u540e\u6765\u7684Cas9\/RNA\u4ea4\u4e92\u4f5c\u7528\u3002crRNA\u4e0etracrRNA\u53ef\u4ee5\u878d\u5408\u6210\u5355\u94fe\u5f15\u5bfcRNA\uff08sgRNA\uff09\u8fa8\u8bc6\u76ee\u6807\u5e8f\u5217\uff0c\u88ab\u8ba4\u4e3a\u662f\u4ed6\u548c\u590f\u5f6d\u63d0\u8036\u535a\u58eb\u7684\u5171\u540c\u529f\u52b3\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; font-size: 17px; line-height: 25px; font-family: 'MS Song', 'MS Hei'; padding: 0px 40px 12px 0px;\">\u5f20\u950b\u535a\u58eb\u76ee\u524d\u4efb\u804c\u9ebb\u7701\u7406\u5de5\u5b66\u9662\uff08MIT\uff09\uff0c\u662f\u9ea6\u6208\u6587\u8111\u79d1\u5b66\u7814\u7a76\u6240\uff08McGovern Institute for Brain Research\uff09\u7684\u7814\u7a76\u5458\u3001\u535a\u5fb7\u7814\u7a76\u6240\uff08Broad Institute\uff09\u7684\u6838\u5fc3\u6210\u5458\uff0c\u4e14\u5728MIT\u62c5\u4efb\u8111\u4e0e\u8ba4\u77e5\u79d1\u5b66\u53ca\u751f\u7269\u5de5\u7a0b\u526f\u6559\u6388\u3002\u4ed6\u662f\u4e00\u4f4d\u534e\u88d4\u7f8e\u7c4d\u4e4b\u751f\u7269\u5de5\u7a0b\u5b66\u5bb6\uff0c\u81f4\u529b\u4e8e\u5f00\u53d1\u5de5\u5177\u6765\u7814\u7a76\u795e\u7ecf\u79d1\u5b66\u4e0e\u4eba\u7c7b\u75be\u75c5\u3002\u4ed6\u5148\u524d\u7684\u7814\u7a76\u9020\u5c31\u4e86&#8221;\u5149\u9057\u4f20\u5b66&#8221;\u79d1\u6280\uff0c\u5e94\u7528\u5728\u795e\u7ecf\u7ec6\u80de\u7684\u518d\u751f\u3002\u5728CRISPR\u6280\u672f\u7684\u53d1\u5c55\u4e2d\uff0c\u5176\u4e3b\u8981\u7684\u8d21\u732e\u5728\u4e8e\u7387\u5148\u4f7f\u5176\u9002\u7528\u4e8e\u4eba\u4f53\u7ec6\u80de\u3002<\/p><p style=\"-webkit-font-smoothing: antialiased; 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